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Antibodies were incubated using an automatic IHC staining instrument (Ventana, Tucson, AZ, USA) according to the manufacturers protocols

FA is caused by mutations in the frataxin gene ( FXN ), which results in a deficiency of the mitochondrial protein frataxin (FXN) [8]

However, the extent to which NRF2 regulates its target genes beyond promoter-restricted regions, and the significance of this regulation to NRF2-dependent cancer cell maintenance, remains unexplored

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The statistical software "Primer of Biostatistics, version 3.02" was used