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4.13.2 Mechanical stress Mechanical testing demonstrates increased tissue stiffness that limits adipocyte expansion and triggers mechanotransduction through focal adhesion complexes (327, 328)

Live imaging microscopy of adipocytes transduced with an Akt sensor (FoxO1-Clover) demonstrated that FoxO1 translocation from the nucleus to the cytosol was reduced, indicting less Akt activation in Mg 2+ -deficient adipocytes

Ethics declarations Competing Interests The authors declare that they have no competing interests

Under diabetic conditions, DAG accumulation occurs through multiple pathways

This is particularly intriguing, as the recent work uncovered that U1-snRNP may serve as a governing machinery for blocking premature polyadenylation 48 , suggesting FUS/TLS might play an important role in regulating polyadenylation selection

These increase methylation demand and interfere with folate metabolism