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On the other hand, our 2-piece d-monobody synthesis strategy has the advantage that the C-terminal peptide contains the FG loop and the randomized library residues of the C/D strands, both major contributors to monobody-target binding, whereas the N-terminal peptide only contains that CD loop, which often is not involved in target binding

Pure peptide aggregation appears as uniform cloudiness or fine particulates suspended evenly throughout the solution, with no colour shift or smell

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That means starting with a low dose and gradually increasing it over several weeks