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For example, PI3K inhibitors (such as wortmannin), AKT inhibitors (such as capivasertib), and mTOR inhibitors (such as rapamycin) have demonstrated potential in preclinical studies to alleviate disease progression by restoring autophagic function ( 4.2.2 AMPK/ULK1 signaling pathway AMPK is a highly conserved serine/threonine protein kinase that functions as a central sensor of cellular energy status, playing a crucial role in coordinating metabolic adaptation and autophagic regulation

For example, reductions in Ruminococcaceae and increases in Paraprevotella have been associated with greater disease severity (Guo et al., 2023)

Severe acute exacerbations and mortality in patients with chronic obstructive pulmonary disease

Therefore, we specifically analyzed the effect of HFD on the lipid metabolism-related transcriptome of MGs and the content of lipid droplets accompanying temporal oscillations