Abnormal accumulation or deficiency of trace elements plays a significant role in cancer pathogenesis and progression, with dysregulation of copper metabolism being particularly notable (143,144)
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Exploring disease through metabolomics
Relf M, LeJeune S, Scott PA, Fox S, Smith K, Leek R, et al
By ~24 hours, the resolution
Briefly, 50 l of nematode supernatant was added to each well of a 96-well plate and 50 l of 100 M DCFH-DA solution (a fluorescent probe) (Beyotime Institute of Biotechnology) was added to give a final DCFH-DA concentration of 50 M, The solutions were thoroughly mixed by shaking for 30 sec
