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Enzyme-like activity of GSH-CDs The superoxide dismutase (SOD)-like activity of the GSH-CDs in removing superoxide anions (O 2 ) was evaluated according to the instructions of the total superoxide dismutase assay kit with nitrogen blue tetrazole (NBT)

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Fluorescence signals were measured at 500 nm excitation and 525 nm emission for intracellular ROS levels, and at 495 nm excitation and 515 nm emission for intracellular RNS levels, using multi-mode microplate reader (SpectraMax iD3, Molecular Devices, USA)

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For example, in patients with metastatic breast cancer, bevacizumab (Avastin), a humanized anti-VEGF monoclonal antibody, has been found to regulate the vasculature, restore DC maturation, and diminish Tregs [75,78]

But McMullen from the AMA urges caution