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Oncogene 18 , 77197730 (1999)

J.ClaushuisT

Vit C prevented the death of human dopaminergic neurons following glutamate neuroexcitotoxicity induced by stimulating -amino-3-hydroxy-5-methyl-4-isoxazole propionic acid and metabotropic receptors, and N-methyl-d-aspartate and kainate receptors in in vitro study (Figure 2B) (99, 100)

Chain elongation of the intermediate is mediated by a condensation (C) domain catalyzing the nucleophilic attack of the downstream PCP-bound acceptor monomer on the activated thioester of the upstream PCP-bound donor monomer 13 , thus giving rise to the dipeptide intermediate D- O -Leu-D-Ala- S -PCP

doi: 10.1038/nature23475 74 KremerDMNelsonBSLinLYaroszELHalbrookCJKerkSAet al

Cells were maintained in DMEM (HCT116, SW480, U2OS), MEM- with 1 ng ml 1 fibroblast growth factor 2 (Peprotech, hMSC) or DMEM with 1% non-essential amino acid (Gibco) and 50 g ml 1 uridine (Sigma-Aldrich) supplementation (143B), with 10% dialysed foetal bovine serum (dFBS), 50 U ml 1 penicillin and streptomycin (ThermoFisher, 15070063) and 1 mM sodium pyruvate (ThermoFisher, 11360070)