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Once in the cytosol, Glu and Cys are combined into the dipeptide -glutamylcysteine (-GC) by the enzymatic activity of glutamatecysteine ligase (GCL), which requires ATP and Mg 2+ as co-substrates (Figure 2A) [15,16,17]

Xia N, Daiber A, Forstermann U, Li H

Bruker NMR ( 1 H at 800 MHz)

GPX4 specific activity was carried out in a coupled assay, by monitoring the consumption of NADPH in the presence of glutathione reductase upon addition of phosphatidyl choline hydroperoxide (Roveri et al., 1994)

Lysosomal calcium signalling regulates autophagy through calcineurin and TFEB

Safety : Generally well-tolerated, with rare side effects under medical supervision