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For secondary staining, the sections were washed three times for 5 min in PBS and incubated for 1 h at room temperature with secondary antibodies dissolved in blocking buffer

"Glucagon-like peptide I stimulates insulin gene expression and increases cyclic AMP levels in a rat islet cell line"

AMPK activation is the same pathway triggered by exercise, caloric restriction, and drugs like metformin

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Brieger K, Schiavone S, Miller FJ, Krause KH (2012) Reactive oxygen species: from health to disease

oleifera modulates immune responses by enhancing the release of Treg cells, cortisol, adrenaline, NK cells, and leptin (Abdel-Latif et al