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Plasmid purification 3XFlagNICD1 was a gift from Raphael Kopan (Addgene plasmid # 20183

Christofi [email protected] This article was submitted to Gastrointestinal and Hepatic Pharmacology, a section of the journal Frontiers in Pharmacology Disclaimer All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers

METALLURGY C07ORGANIC CHEMISTRY C07CACYCLIC OR CARBOCYCLIC COMPOUNDS C07C227/00Preparation of compounds containing amino and carboxyl groups bound to the same carbon skeleton C07C227/38Separation

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The reaction mixture was retrotranscribed at 48 C for 45, denatured for 2 at 94 C and then subjected to 1826 cycles of denaturation for 30 seconds, annealing at 55 C for 45 seconds, and elongation at 68C for 1 minute