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Cells (2021) 10(5):1236

Briefly, in the NADH-coupled assay, 14 g (1.2 M) of detergent-purified protein was added to 150 L of reaction buffer containing 50 mM HEPES-KOH pH 8.0, 60 g/mL pyruvate kinase (Roche), 16 mg/mL lactate dehydrogenase (Roche), 4 mM phosphoenolpyruvate (Roche), 0.3 mM NADH, 3 mM ATP, 10 mM MgCl, 0.01% (w/v) LMNG, and 0.001% (w/v) CHS

Proteomic profiling of leukocytes reveals dysregulation of adhesion and Integrin proteins in chronic kidney disease-related atherosclerosis

Magnsdttir S, Ravcheev D, de Crcy-Lagard V, Thiele I

For consistency in research, it's the most reliable method

This means that taking massive, single doses (e.g., 20 grams all at once) does not proportionally increase blood plasma concentrations, as the specific intestinal transporters simply become overwhelmed and cannot process the excess