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Caution: May interact with substances that affect antioxidant balance

Cell culture RAW 264.7 murine macrophage cells (TIB-71, American Type Culture Collection) were cultured in DMEM (4.5 g/L d-glucose) supplemented with 10% (vol/vol) FBS, 100 units/mL penicillin and streptomycin (Gibco) at 37 C with 5% CO 2 in humidified air as previously stated in 21

and 13000 g for 15 min, and the resulting supernatants processed using established protocols

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