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& Howlett, B

Bonferroni analysis was used as an additional/secondary result to visualize differences in glutathione levels according to risk severity

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Liver samples were then mixed with 4X Laemmli sample buffer (8% SDS, 40% glycerol, 0.4% bromophenol blue, 240 mM Tris, pH 6.8) with freshly added -mercaptoethanol and were heated for 5 mins at 95 C