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This shift may result from the saturation or downregulation of glycolytic pathways, reflecting an adaptive response to prolonged oxidative stress and sustained strain of the metabolic pathways (Mussi et al

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The resulting upper (lipids) and middle (metabolites) phases were collected separately in 1.5 mL glass vials and dried out using SpeedVac Concentrator (Thermo Fisher Scientific, SC110A)

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