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Bacteria were grown in Luria-Bertani (LB) broth supplemented with 40 g/ml kanamycin and 25 g/ml chloramphenicol at 37 C for 5 h and induced overnight at 20 C by the addition of 50 M IPTG and 0.5 mg/ml L-arabinose

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Then the cell co-culture model was employed to examine the bone marrow-derived dendritic cells (BMDCs) maturation in vitro with the aid of R848 after SDT

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