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(B) Zoomed-in view detailing the distance between the sulfur atoms of Cys128 and Cys150

Progranulin derived engineered protein Atsttrin suppresses TNF--mediated inflammation in intervertebral disc degenerative disease

Metabolism 51(11):13891391

70 mL of 10 % HCl was added to the resulting concentrated solution which was then heated and stirred at a temperature of 80 to 90 0 C for 2 hours, concentrated by distillation under reduced pressure, and dissolved in a small amount of water, followed by elution on a column packed with an anion exchange resin (Amberlite IRA 410, OH " )