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Determination of barrier properties of the cells particularly tight junction tightness and functional expression of polarized membrane transporters are carried out to evaluate the goodness of purpose of the models

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Alpha-glucosidase inhibitory peptides: sources, preparations, identifications, and action mechanisms

Hand Clin 16:6772

Engineered as a synthetic peptide based on the native GIP sequence and modified for extended half-life via fatty acid conjugation, tirzepatide delivers glycemic control, profound weight loss, and cardiometabolic benefits

48 h after transfection, cells were washed twice with pre-warmed PBS and fixed with 4% paraformaldehyde (PFA) for 1015 min