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Mechanistically, Saracatinib inhibits TGF--induced Smad3 phosphorylation, thereby suppressing key fibrotic markerssuch as -SMA, type I collagen, and plasminogen activator inhibitor-1 (PAI-1)in human lung fibroblasts, while reversing EMT-related pathways (Hu et al., 2014

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Sixty minutes before the beginning of the behavioral testing, 0.2 mL of 0.1 mg mL 1 clozapine-N-oxide dissolved in saline (CNO, Sigma-Aldrich, cat#C0382) or saline was administered intraperitoneally to the subject female mice, and pups, food, and water were removed