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Immediately prior to infection, the temperature setpoint was either maintained at 37C or changed to a range of temperatures between 31C and 38.5C across multiple experiments with each condition performed in duplicate (Table 1

Lysates were clarified by centrifugation, and the resulting supernatant was incubated with 1 mL of Ni-NTA Agarose (Qiagen, 30210) for 1 h at 4 C

FIGURE 1 2.2 Search strategy A systematic search of scientific literature was conducted in the Web of Science (Core Collection), Scopus, and PubMed databases, including studies published up to November 2025 without country restrictions

Gene therapy directly addresses the genetic cause of HCU, offering the possibility of durable metabolic correction

Phase III (transport and elimination) involves specialized transport proteins to pump the phase II conjugated toxins out of the cells

Furthermore, sesamin-treated mice maintained higher levels of endogenous antioxidants for example, superoxide dismutase (SOD) activity was increased relative to untreated MPTP mice, and striatal TH levels were better preserved, indicating reduced dopaminergic neuron damage (66)