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Local anesthetics, such as lidocaine and bupivacaine, reversibly block voltage-gated sodium channels in excitable membranes, preventing action potential initiation and propagation along sensory neurons [60,61,62,63]

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From each sample 100 g protein was mixed with 4x Laemmli buffer (277.8 mM Tris-HCl pH 6.8, 26.3 % (w/v) glycerol, 2.1% SDS, 0.01 % bromphenol blue (Na-salts), 40 mM NEM) for non-reducing condition and with 4x Laemmli buffer plus 20% (v/v) 1 M DTT for reducing condition