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Organization of the laminin polymer node

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(2020, September 17)

For the in vitro interaction, 1 g of recombinant histone H1.0 was either incubated with 5 g of Flag-SSRP1, Flag-SSRP1 mutants or Spt16 and Flag-SSRP1 (5 g each) in the FLAG-IP buffer (50 mM Tris-HCl pH 7.4, 200 mM NaCl, 1 mM EDTA, 1% Triton X-100) supplemented with fresh protease inhibitors (Millipore) for 2 h on a rotating wheel at 4 C

Compared to free EOs, NCs containing EOs from Rosmarinus officinalis L

The most severe type of sperm DNA damage is double-strand break, and it will be repaired by DSBR, including homologous recombination (HR), classical non-homologous end joining (cNHEJ), alternative end joining (aEJ), and single-strand annealing (SSA)