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: Proteolysis-inducing factor is expressed in tumours of patients with gastrointestinal cancers and correlates with weight loss

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After protein measurement using the Bradford assay [41], 10 g of proteins from each sample were introduced and subjected to SDS-PAGE separation prior to being transferred to a PVDF membrane and blocked with 5% bovine serum albumin (BSA)

Cytokine array The cytokine assay was performed using a Proteome Profiler Mouse Cytokine Array Kit (R and D systems, Minneapolis, MN, USA )

OROS lyophilized products are formulated, sterile filled, lyophilized, vialed, and packaged in California at a qualified third-party manufacturing facility operating under controlled processes aligned with cGMP principles