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Indeed, the determination of the perinuclear mitochondrial CMFDA staining after co-localization with mitotracker red (as described previously [24]) (figure 4C) in 3T3 fibroblasts at 24 hours of culture show that BSO treated cells maintained a high mitochondrial CMFDA staining but cytosolic fluorescence was indeed decreased by BSO

Bioinformatics 27, 15711572

Moreover, as cystine is an extracellular source of cysteine, cystine uptake is facilitated by the efflux of glutamate via the xCT antiporter

Hoagland et al

Notably, RT-qPCR analysis showed a sharp increase in OCT4 mRNA expression, a marker of pluripotent stem cells and essential for TIC self-renewal [33], in the colonies formed in 3D gels relative to 2D culture conditions (Fig