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Effect of reduced glutathione (GSH) in canine sperm cryopreservation: in vitro and in vivo evaluation

However, NAC appears to have a cell type-specific action: in human bronchial epithelial cells, NAC inhibited silica-induced NFkB at a concentration of 5 mM [60]

Recent findings indicate that BCP alleviates oxidative stress, inflammation, and apoptosis, demonstrating a protective effect against doxorubicin-induced cardiotoxicity (Meeran et al., 2019)

However, it is essential to note that GSH levels in both dose groups generally returned to baseline levels after a one-month washout period, suggesting that continuous supplementation is necessary to maintain elevated GSH levels

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Secondary antibodies were added in 10 ml of blocking buffer and incubated with membrane with gentle agitation for 1 hour at room temperature