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Ten micro liters of the supernatant which was filtered through a 0.2 m filter was mixed with 100 l of derivatization reagent (prepared by dissolving 10 mg o-phtaldialdehyde in 0.5 ml of methanol, 2 ml of 0.4 M borate buffer (pH 10.0) and 30 l of 2-mercaptoethanol) and then injected into the chromatographic system

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Selenium serves as the active center in selenoproteins and plays a key role in glucose metabolism and redox processes in animals [58]