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Bacteria were incubated at 37 C, 140 rpm, microaerophilic conditions, 5% CO 2 , 10% O 2 and 85% N 2 and passaged in 72-h intervals in the presence of metronidazole, MF-01 or Metro-P3 at different concentrations (0.25, 0.5, 1, 2 MIC)

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Limitations and challenges in clinical translation Although this study has confirmed that AAP NPs can effectively inhibit COC by targeting and inducing ferroptosis, their limitations and the severe challenges in clinical translation must be fully recognized and elaborated

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with Q-dots (red) were imaged 5 minutes following a normal thinned skull preparation (control