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Later experiments have found that adding high doses of copper can increase reactive oxygen species and protein carbonylation levels, decrease glutathione levels and SOD enzyme activity, leading to depolarization of mitochondrial membrane potential, the release of cytochrome c, elevated levels of active caspase-9 and caspase-3, increased levels of Bcl2-antagonist/killer 1 (Bak1) and Bcl2-associated X protein (Bax), and decreased levels of B-cell lymphoma 2 (Bcl2), ultimately resulting in cell apoptosis (134)

10.1016/j.transci.2023.103755 78 RinalducciS.FerruE.BlasiB.TurriniF.ZollaL

Itsumura N, Kibihara Y, Fukue K, Miyata A, Fukushima K, Tamagawa-Mineoka R, et al

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Enriching zinc consumption is crucial for the body to have a healthy antioxidant system

In additional, our controls were not given a placebo