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Membranes were excised and incubated in phenol red-free Williams Medium E medium (PAN-Biotech) containing 100 M monochlorobimane (mBCI) (Sigma-Aldrich), 5 M CellROX Deep Red Reagent (Thermo Fisher Scientific) and 200 nm MitoTracker Red CMXRos (Thermo Fisher Scientific) for 30 min at 37 C and 5% CO 2

What causes inflammation of this nature are things that place an excessive stress load on the body

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