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Redox-state analysis DF-1 cells were lysed by freezethaw method, and reduced/oxidized glutathione (GSH/GSSG) measurement was performed using a GSH and GSSG Assay Kit (Beyotime) according to the manufacturers protocol

this indicates that there is some flexibility in nutrient usage, which is crucial to support cellular fitness (Fig

OConnor JC, McCusker RH, Strle K, Johnson RW, Dantzer R, Kelley KW: Regulation of IGF-I function by proinflammatory cytokines: at the interface of immunology and endocrinology