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The C-terminal active site cysteine of Escherichia coli glutaredoxin 1 determines the glutathione specificity of the second step of peptide deglutathionylation

Briefly, the cells, plated at 5 10 5 in a 35 mm diameter dish and cultured for 24 h, were rinsed three times with PBSG and incubated in the dark at room temperature for 10 min with 100 l of 8 mM monobromobiamine in 50 mM N -ethylmorpholine, pH 8, and 100 l of PBSG

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[PubMed: 11713213] 73

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