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Mg 2+ binds to ATP, forming the MgATP 2 complex, which is the true substrate of the enzyme rather than free ATP

The underlying mechanism of microglial activation induced by METH is related to the Toll-like receptor 4 (TLR4) located on microglia, which is involved in the immune surveillance of pathogens and exogenous small molecules (Bachtell et al., 2015)