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The samples of blood/liquor were collected in rated intervals (see experimental design) from the jugular vein or cisterna magna respectively, via the polyethylene tubing (BTPE10, Instech, USA) applied before the glutamate application

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At the molecular level, zinc deficiency downregulates TJ proteins such as claudin-3 and occludin, leading to increased permeability and compromised barrier integrity (Zhong et al., 2010)

Mock-treated samples were supplemented with an equivalent volume of DMSO

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Nevertheless, a subset of patients continues to face therapeutic challenges associated with primary/secondary treatment failure, adverse reactions, and disease heterogeneity, which has driven the development of emerging therapies based on pathological mechanisms