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In our GST purification protocol, we suggest using 1x PBS, pH 7.4 as the binding buffer

Figure 2 It has been hypothesized by certain researchers that there is an interaction between retinal macrophages and ferroptosis, given the resemblance in the functions of the macrophages and the traits of ferroptosis ( Macrophages phagocytosis contributes considerably to the removal of ferroptotic cells, which could release a DAMP called HMGB1 to activate and recruit macrophages, alerting immune defenses ( In the context of ferroptosis, either advanced glycosylation end-product specific receptor (AGER) or Toll-like receptors (TLR) on macrophages is required for DAMPs to mediate inflammation

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Bauer M, Herbarth O, Aust G, Hengstler JG, Dotzauer A, Graebsch C, et al

10.1104/pp.111.181875 13 ChenZ.GallieD