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These observations were further supported by quantification, which indicated a decrease in the percentage of dead and apoptotic cells in Atp7b / :Prnp / hepatocyte populations compared to hepatocytes isolated from Atp7b / mice (Supplementary Fig

However, the effect of oxidative and nitrosative stress is not restricted to the intracellular environment, as reactive species can cross cellular membranes and influence extracellular proteins

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Directed evolution of LaccID: generation 2 For the second round of evolution, three libraries were generated using G1 (LacAnc100 + R121S) as the starting template