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Consistently, there was no significant change in the total contributions of U 13 C-glucose to glutamate (Fig

Cytokines Measurement The levels of TNF- and IL-1 in serum and peritoneal lavage fluid collected at 24 h post CLP induction were quantified by Enzime-Linked Immunosorbent Assay (ELISA) using an appropriate commercial kit (R&D Systems, Minneapolis, Canada) according to the manufacturer's instructions

However, sustained production of pro-inflammatory cytokines and chemokines can lead to disruption of immune homeostasis

Monocyte chemoattractant protein-1 is produced in isolated adipocytes, associated with adiposity and reduced after weight loss in morbid obese subjects

Real-Time PCR Total RNA of kidney tissue was extracted by TRIzol method

DC, dendritic cells