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After the final heat-shock at 65 C the samples were centrifuged at 20,000 g and 4 C for 10 min

This review consolidates recent findings indicating that FOXO4-dependent senescence significantly contributes to neuroinflammation, synaptic dysfunction, and impaired neurogenesis in the aging brain

We use clinical photography (front, three-quarter, profile, under cross-polarized light) to document the baseline, the four-week milestone, and the three-month outcome so progress is measurable rather than felt