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These fractions were examined by analytical thin layer chromatography (TLC) using silica gel 60 PF 254 precoated plates (Whatman) and several solvent systems

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This releases a large number of tumor antigens and DAMPs to activate DCs [260,261,262]

Epitomics), The membranes were washed three times in TBST for 5 mins each and were incubated at room temperature for 1 hr with horseradish peroxidase conjugated rabbit anti-mouse or anti-rabbit IgG secondary antibody (Sigma-Aldrich, anti-mouse Cat

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