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Each PCR product was sub-cloned into pDONR-Zeo (Invitrogen) or pGEM-T Easy vectors (Promega) for further experiments

Fe-np was of special interest because in-situ lunar regolith has been observed to contain Fe-np due to meteorite impacts but are not included in the lunar simulant

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Therefore, we defined the MTD of DKFZ-608 as 15 mg/kg, which is the dose that was used for tumor treatment