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For each PCR condition, SYBR Green I Master (Roche Diagnostics, Mannheim, Germany) and the following primers were used: FTL: 5-TACGAGCGTCTCCTGAAGATGC-3 and 5-GGTTCAGCTTTTTCTCCAGGGC-3, PTGS2: 5- ATATGTTCTCCTGCCTACTGGAA -3 and 5-GCCCTTCACGTTATTGCAGATG-3, ACSL4: 5- TGGAAGTCCATATCGCTCTGT -3 and 5- TTGGATACAGCATGGTCAAA-3, HMOX-1: 5- CAACATCCAGCTCTTTGAGGA -3 and 5- T GGGCAGAATCTTGCACTTTG-3, Nrf-2: 5- TACTCCCAGGTTGCCCACA-3 and 5- CATCTACAAACGGGAATGTCTGC-3, GSTP1: 5- TACACCAACTATGAGGCGGG-3 and 5- AGCGAAGGAGATCTGGTCTC-3

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However, with consistent use, many people notice improvements in their skins hydration, texture, and overall appearance within a few weeks to a few months