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Ultraperformance liquid chromatographymass spectrometry (UPLC-MS) analysis of Kratom extracts Untargeted metabolomics analyses were conducted using an Acquity Ultra-Performance Liquid Chromatography system (Waters, Milford, MA, USA) coupled to a Q Exactive Plus Hybrid Quadrupole-Orbitrap mass spectrometer (Thermo Fisher Scientific, Waltham, MA)

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In addition, in the B12 deficient group, biochemical parameters such as folate (22.4 vs 14.9 nM) and HDL cholesterol (1.4 vs 1.7 mM) levels were significantly lower, whereas TG (3.5 vs 2.9 mM) was higher