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Since thioredoxin is readily oxidized, it has to be reduced using thioredoxin reductase before it is used for experiments

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The appropriate buffer was then used to dissolve SAM from stocks to 500 M

Apart from glycolysis in cancer cells, glutaminolysis (i.e., a series of biochemical reactions by which glutamine is lysed to glutamate) is another main pillar for energy production, including the conversion of glutamine to -ketoglutarate (KG), reaction steps of the citric acid cycle and malate aspartate shuttle, and the conversion of malate to pyruvate and lactate