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Self cleaving affinity tags based on inteins. A, initial tags used Download Scientific Diagram Facile expression of proteins with desired N terminal amino acid via an engineered cysteine protease domain Communications Biology glutathione intein cleavage Recent advances in in vivo applications of intein mediated protein splicing Mobile DNA Manual Solid Phase Synthesis of Characterization of a self splicing mini intein and its conversion into autocatalytic N and C terminal cleavage elements: facile production of protein building blocks for protein ligation ScienceDirect glutathione intein cleavage A) A proposed mechanism for thiol induced reactions at the FRET based N extein cleavage assay. (A) Frontiers The Evolution of Intein Based Affinity Methods as Reflected in 30 years of Patent History
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