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CagriSema is two separate peptides, semaglutide and cagrilintide, combined in one injection.

Mixing two peptides

aCSF was removed by careful suctioning and replaced with aCSF containing vehicle (aCSF+aCSF), 100 ng/ml with carrier free adult recombinant Hepatocyte Growth Factor (HGF) (R and D Systems, MN, USA) in aCSF, 10 10 M Hinge (Harding lab), 50 ng/ml in aCSF, 10 10 M Dihexa (Harding lab) in aCSF, 10 12 M Dihexa in aCSF or 50 ng/ml HGF+10 12 M Dihexa in aCSF for 30 minutes at 37 C

The samples were then centrifuged at 2,000 g for 15 min at 4C to separate the serum

doi: 10.1161/CIRCULATIONAHA.116.026099

With no airspace in the IV line, you can start inserting the cannula into the patients vein