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Meng J, Zhang Q, Yang C, Xiao L, Xue Z, Zhu J

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The protein pellet was analyzed using Pierce BCA Protein Assay kit (Thermofisher) to determine protein concentrations, and the supernatant was transferred into a new tube for the identification of metabolite levels by mass spectrometry analysis at the Metabolomics Expertise Center (MEC - KU Leuven/VIB)

Gene expression analysis Using the RNeasy-Mini Kit (Qiagen Inc., Valencia, CA, USA), total RNA from bladder tissues was isolated, and the DNA-free Kit (Applied Biosystems, Foster City, CA, USA) was used to remove genomic DNA

After that, the eluate is dialyzed against, for example, buffered saline in order to remove the reagent used for elution

Jul 28 2025;doi:10.1001/jama.2025.12923