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doi: 10.1038/nrmicro1324 72 DelbazA.ChenM.JenF

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Western blot analysis involved preparing whole cell lysates using RIPA lysis buffer, quantifying protein concentration via BCA assay, followed by SDS-PAGE, transfer to nitrocellulose membranes, incubation with primary and HRP-conjugated secondary antibodies, and detection using an ECL solution [1]

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Blots were visualized using a chemiluminescence system (Bio-Rad, Hercules, USA)

Moderna is also preparing to ask the FDA to authorize its bivalent vaccine that targets BA.4 and BA.5 as well as the original strain