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Under normal physiological conditions, ROS are produced as byproducts of cellular metabolism through processes such as mitochondrial respiration, enzymatic reactions, and immune responses [7-9]

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To ensure that the Wolbachia were viable, and not dead with an intact membrane, drug-treated extracellular Wolbachia were then incubated with C6/36 cells to assess their ability to reinfect

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In contrast, combined LT4 and liothyronine (LT3) therapy may offer symptomatic improvement in selected patients, though robust criteria for selection remain undefined