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B-mode ultrasound was used to determine the locations of the liver capsule and overlying tissue (muscle, fat, and skin), followed by a TA procedure consisting of 10 to 30 measurements consisting of 80 pulses of 1-microsecond Radio Frequency (RF) pulses, each containing 5.7 milliJoules of energy

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The standard pellet diet comprised 21% protein, 5% lipids, 4% crude fibre, 8% ash, 1% calcium, 0.6% phosphorus, 3.4% glucose, 2% vitamin and 55% nitrogen free extract (carbohydrates)

These studies further revealed that GSH depletion specifically impacted mitochondrial genome stability rather than nuclear genome stability, highlighting the essential requirement for GSH in mitochondrial metabolism