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Determination of enzyme activity of GSH, GR, APX, GST, and GPX GST, GPX, GSH, GR, APX were measured using GST Assay Kit, GPX Assay Kit, GSH Assay Kit, GR Assay Kit, and APX Assay Kit, respectively, according to the manufacturers instructions from the Beijing Solarbio Science & Technology (Beijing, China)

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50 L of sample was mixed with 0.5 mM CuSO4, 1 mM BTTAA, 100 M Rhodamine-Azide, and 2.5 mM freshly prepared sodium ascorbate for 2 hours at 25 C

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When a deficiency is present, some people notice brain fog, and correcting low levels can help with focus and mental clarity