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Following IVM, oocyte maturation was scored, with GV oocytes being identified by the presence of a nuclear envelope and nucleolus, MI oocytes identified by the absence of the nuclear envelope and nucleolus, MII oocytes identified via the presence of the first polar body, and degenerative oocytes identified via cytoplasmic fragmentation 106, 107

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Hemochromatosis protein (HFE) knockout mice as a novel model of hemochromatosis: implications for study and management of iron-overload cardiomyopathy