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Determination of superoxide, hydrogen peroxide, root membrane injury and NADP(H)-oxidase Superoxide (O 2 ) content of the tissue was determined from plant tissue homogenized in 65 mM phosphate buffer (pH 7.8) and supernatant were collected by 8,000 g for 30 min, 4 C

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Acknowledgments The authors thank colleagues from their institutions for proofreading the manuscript